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KMID : 0370220210650020065
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Yakhak Hoeji
2021 Volume.65 No. 2 p.65 ~ p.71
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Effects of 20(R)-Ginsenoside Rg3 and 20(S)-Ginsenoside Rg3 on the migration and invasion of HaCaT cells
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Kim Sang-Won
Noh Young-Chan
Park So-Yeon
Lee Mi-Jin
Choi Hui-Ju
Song Gyu-Yong
Kim Han-Gun
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Abstract
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Wound healing in the skin is a complex process involving several steps. First is the inflammation stage, in which the wound causes constriction of damaged blood vessels and the aggregation of platelets, aiding neutrophils and macrophages in removing the damaged tissue and preventing infection. This is followed by the proliferation stage involving keratinocytes and fibroblasts. Finally, in the tissue remodeling stage, the wound healing process is completed through contraction of the wound area, which promotes the growth of new tissue. In particular, the migration of keratinocytes and fibroblasts during the proliferation stage to the wound site and penetration of these cells into the surrounding tissues promote the wound healing process. In this study, we performed MTT assays to evaluate the toxicity of 20(R)-Rg3 and 20(S)-Rg3, stereoisomers of ginsenoside Rg3, an active ingredient of Korean ginseng, on the keratinocytes and fibroblasts involved in the proliferation stage. Migration assays, invasion assays, and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to assess the expression of genes related to wound healing. We observed that 20(R)-Rg3 was non-toxic and promoted the expression of COL1A2 and ACTC1, genes related to wound healing, thereby increasing the mobility and invasiveness of keratinocytes. In addition, we confirmed that 20(R)-Rg3 increased the initial rate of wound healing in vivo in BALB/c mice. The positive effect of 20(R)-Rg3 on wound healing can be assessed by separating this isomer from 20(S)-Rg3, which has moderate toxicity against keratinocytes and reduces mobility and invasiveness. Thus, 20(R)-Rg3 optical isomers could be potential candidates for the development of effective wound healing medicines.
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KEYWORD
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Ginsenoside Rg3, 20(R)-Rg3, HaCaT, Wound healing
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